A three-year quest to find the giant Chinese paddlefish in the Yangtze river failed to sight or catch a single individual, according to a BBC report.
That means that the fish, which can grow up to seven metres long, has not been seen alive for at least six years.
There remains a chance that some escaped the survey and survive, say experts, but without action, the future of the species is bleak.
The concern for the Chinese paddlefish is that its fate will parallel that of the Yangtze river dolphin, a large mammal species that was once abundant in the Yangtze river system but has recently been declared extinct.
A number of fish species vie for the position of the world's largest freshwater fish, including the arapaima (Arapaima gigas) of the Amazon river and the Mekong giant catfish (Pangasianodon gigas).
At up to 7m, the Chinese paddlefish (Psephurus gladius) is much longer than either, though it may not exclusively live in freshwater.
The fish is suspected to be anadromous, meaning it spends some of its life in marine waters before returning to the river to spawn. But it is so rare that little is known about its behaviour, life history, migration habits and population structure.
It is endemic to the Yangtze river system in China.
Giant Chinese Fish Thought Extinct
CHINA - One of the world's largest freshwater fish, the Chinese paddlefish, is on the verge of becoming extinct.