Aleksei Krasnov is on the far right © David Fields
The encounter between a salmon louse and its host begins when the louse is in its free-swimming larval stage, known as a copepodite. But being tiny organisms in a vast ocean, how do they locate salmon as their host?
"Chemical signalling is believed to play a key role in host-parasite communication, and scientists have confirmed this," says Nicholas Robinson, a senior scientist at Nofima.
Robinson is coordinating the CrispResist project, which brings together a leading team of scientists from Norway, the UK, USA, Canada, Sweden and Australia. Their goal is to uncover the mechanisms behind cross-species variation in host resistance to sea lice and apply this knowledge to enhance Atlantic salmon resistance in aquaculture.
A crucial objective is to identify and document genes and mechanisms responsible for the difference in sea lice resistance between salmonid species. It is well-established that certain Pacific salmon species are resistant to sea lice and can kill them in the early stages of parasitism, whereas Atlantic salmon are highly susceptible.
Aleksei Krasnov, a senior fish health scientist at Nofima, is involved in studies of chemical communication between lice and salmon. Working with the global team, Krasnov identified putative semiochemicals or compounds of biological origin that affect the behaviour of animals of the same or other species. The type of semiochemicals called kairomones help lice find salmon by scent.
Testing lice behaviour
The discovery involved a wide range of chemical analyses and lice behaviour tests. Water that was conditioned with Atlantic salmon, Pacific salmon and other fish species was analysed. Twenty-one candidate semiochemicals were selected for lice behaviour tests.
Additionally, mucous from Atlantic salmon families with high and low resistance to lice was studied to determine whether resistance could be linked to the chemical composition of the mucous. Behavioural tests were carried out in Norway and Sweden using different methods.
The research showed that water conditioned only with salmon stimulated copepodite activity, confirming the presence of kairomones. Interestingly, conditioned water also contained compounds that deterred lice, suggesting that Atlantic salmon can repel lice as well.
Moreover, the tests suggested that semiochemicals may be produced in various tissues of Atlantic salmon, especially the skin.
Salmon from families susceptible to sea lice were found to produce mucous that had a higher stimulatory effect on lice than salmon from families with high resistance to lice.
Future research
One of the key outcomes of the study was the identification of compounds for further research.
"Overall, the findings suggest that host-parasite communication is highly complex and likely involves multiple cues," says Krasnov.
Krasnov believes that developing molecular tests is the most promising approach for continuing semiochemical research in this field.
*CrispResist is financed by the Norwegian Seafood Research Fund – FHF. The project is a collaboration between 12 partners from science and industry. In particular, Rothamsted Research (UK), University of Gothenburg (Sweden), Bigelow Laboratory of Ocean Science (USA) and Nofima (Norway) have contributed to this part of the project.
