Defra has issued a Designated Area Order prohibiting the movement of fish to and from Shirkoak Farm, Woodchurch, Ashford, Kent.
Samples were taken from the affected fishery following reported deaths of fish.
KHV Disease affects common carp and carp varieties such as Koi. It can result in high rates of fish mortalities.
There are no implications for human health.
It became a legal obligation to notify suspicion of KHV Disease on 6 April 2007. The measures developed in partnership between government and industry to help combat outbreaks were announced on 21 May.
Koi Herpesvirus Disease Outbreak Confirmed
UK - Movements of fish in and out of a fishery in Kent have been restricted, following the confirmation of Koi Herpesvirus (KHV) Disease.